Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
HTRF Human Total ULK1 Detection Kit, 50,000 Assay Points
Molecular Devices Luminescence Filter, Long-pass 610 nm, Emission
The Molecular Devices 610 nm LP luminescence filter (cat. #6590-0117) is a long-pass emission filter optimized for luminescence detection and BRET acceptor measurements. It is intended for use as an acceptor/emission filter in NanoBRET assays and is documented for compatibility with filter-based multi-mode microplate readers such as the SpectraMax iD5.
- Provides a long-pass emission cutoff at 610 nm.
- Optimized for luminescence and BRET acceptor detection.
- Designed for use in filter-based microplate reader optical systems.
- Supports assays requiring acceptor emission separation in NanoBRET workflows.
Revvity Health Sciences Inc HTRF dsRNA (IVT) Detection Kit, 100,000 Assay Points
HTRF dsRNA (IVT) Detection Kit, 100,000 Assay Points
Molecular Devices Time-Resolved Emission Filter, 616 nm, 10 nm Bandwidth
This Molecular Devices time-resolved emission filter provides a 616 nm center wavelength with a 10 nm bandwidth for time-resolved fluorescence (TRF) and TR-FRET detection in multi-mode microplate readers. It is intended to isolate emission bands and improve signal selectivity in time-resolved assays.
- Optimized for time-resolved fluorescence and TR-FRET assays.
- 616 nm center wavelength, 10 nm bandwidth.
- Emission filter configured for use in microplate reader filter holders.
- Improves emission selectivity and reduces background signal.
- Referenced in instrument application notes for optimized TR-FRET settings.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD4 (Thr277) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-SMAD4 (Thr277) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF MDM2 Binding Kit, 500 Assay Points
HTRF MDM2 Binding Kit, 500 Assay Points
Electron Microscopy Sciences MAC X-Ray Fluorescence Single Standard Nd
These standards are supplied as 1/4" (33 mm) diameter pressed pellets or metal foils, and are prepared from selected high purity elements and compounds to ensure interference free-spectra. They are suitable for use by experienced spectroscopists as well as staff training. Each of the pressed pellets is supported by a thin-walled aluminum cup which is protected from damage during handling and minimizes the risk of touching the standard materials (some of which are harmful).
Revvity Health Sciences Inc HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, Control Lysate
HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, Control Lysate
Molecular Devices Absorbance Filter, 420 nm, 8 nm bandwidth, FilterMax
Molecular Devices FilterMax absorbance filter designed for fixed-wavelength absorbance assays. The filter provides narrowband transmission centered at 420 nm with approximately 8 nm full width at half maximum and is supplied as an accessory for FilterMax microplate readers.
- Provides narrowband transmission centered at 420 nm.
- Approximately 8 nm full width at half maximum for high wavelength specificity.
- Optimized for absorbance assays on compatible multimode readers.
- Supplied as a replaceable accessory for FilterMax F3 and F5 series readers.
Molecular Devices Fluorescence Filter, 535 nm, 25 nm, With Holder
Molecular Devices fluorescence emission filter with integrated holder provides a narrow 535 nm center wavelength and 25 nm bandwidth for selective detection of fluorophores emitting near 535 nm. It is intended for filter-based microplate readers and supports fluorescence and TR-FRET assay detection, including TAMRA-based assays.
- Center wavelength 535 nm.
- Bandwidth 25 nm.
- Includes integrated holder for installation in filter slot.
- Suitable for fluorescence and TR-FRET detection workflows.
- Designed for use in filter-slot multi-mode microplate readers.
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